Hyaluronic acid-coated chitosan nanoparticles as carrier for the enzyme/prodrug complex based on horseradish peroxidase/indole-3-acetic acid: Characterization and potential therapeutic for bladder cancer cells

Hybrid nanoparticles composed of different biopolymers for delivery enzyme/prodrug systems are interest cancer therapy. Hyaluronic acid-coated chitosan (CS/HA NP) were prepared to encapsulate individually an enzyme/pro-drug complex based on horseradish peroxidase (HRP) and indole-3-acetic acid (IAA). CS/HA NP showed size around 158 nm increase 170 200 after IAA HRP encapsulation, respectively. Nanoparticles positive zeta potential values (between +20.36 mV +24.40 mV) higher encapsulation efficiencies both (up 90 %) obtained. Electron microscopy indicated the formation spherical particles with smooth surface characteristic. Physicochemical thermal characterizations suggest IAA. Kinetic parameters encapsulated similar those free enzyme. IAA-CS/HA a bimodal release profile high initial (72 followed by slow-release pattern. The combination HRP-CS/HA IAA- reduced 88 % cell viability human bladder carcinoma line (T24) in concentrations 0.5 mM pro-drug 1.2 ?g/mL enzyme 24 h.